Orthovanadate increased tyrosine phosphorylation of many proteins.

HCD57 cells were deprived of EPO and treated with 70 μmol/L orthovanadate. Whole cell extracts from 0 (lane A), 1 (lane B), 6 (lane C), 24 (lane D), 48 (lane E), 72 (lane F), and 96 (lane G) were analyzed on a Western blot (WB) probed with an antiphosphotyrosine antibody as described in “Materials and methods.”