Fluorescence in situ hybridization assay for the detection of t(11;18)(q21;q21) on metaphase chromosomes and interphase nuclei.

(A) On the left side, the normal localization of PACs 166G16 and 59N7 can be seen. As shown in panels B and C, t(11;18) leads to a fusion or colocalization signal of both probes on the derivative chromosome 11. (D) PAC 166G16 is split by the translocation leading to either 3 hybridization signals of this probe located on the derivative chromosomes 11 and 18 and the normal chromosome 11 (case 8) or, (E) in the case of a deletion of chromosome 11 sequences, to 2 signals of this probe located on the derivative chromosome 11 and the normal chromosome 11 (case 6).