American Society of Hematology

Fig. 4.

$Fig. 4. Mature DCs cross-present LCL-derived epitopes derived from freeze–thaw lysates to freshly isolated CD4+and CD8+ T cells. / Immature DCs generated from donors IP1 and IP2 were loaded with lysate fractions 10 kd or larger prepared from either donors' EBV B-LCL or B-cell/T-cell blasts. After maturation was induced (see “Materials and methods”), DCs were added to freshly isolated autologous CD4+ and CD8+ T cells in 20-hour IFN-γ Elispot assays. Resulting spots were developed and counted as described in Figure 1. Each bar represents the mean spot number of triplicates ± SD per 105 CD4+ T lymphocytes (▪) or CD8+ T lymphocytes (░) initially seeded per well. Calculation of lysate-responsive T-cell frequencies were performed as outlined in Figure 1. Results were confirmed in 4 independent experiments.$

Mature DCs cross-present LCL-derived epitopes derived from freeze–thaw lysates to freshly isolated CD4⁺and CD8⁺ T cells.

Immature DCs generated from donors IP1 and IP2 were loaded with lysate fractions 10 kd or larger prepared from either donors' EBV B-LCL or B-cell/T-cell blasts. After maturation was induced (see “Materials and methods”), DCs were added to freshly isolated autologous CD4⁺ and CD8⁺ T cells in 20-hour IFN-γ Elispot assays. Resulting spots were developed and counted as described in Figure 1. Each bar represents the mean spot number of triplicates ± SD per 10⁵ CD4⁺ T lymphocytes (▪) or CD8⁺ T lymphocytes (░) initially seeded per well. Calculation of lysate-responsive T-cell frequencies were performed as outlined in Figure 1. Results were confirmed in 4 independent experiments.

This Feature Is Available To Subscribers Only