Coexpression of ATF2 and c-Jun activated ATF3 gene promoter, but ATF3 repressed it.

Cultured HUVECs were transfected with 2 μg of pLuc-1850 (solid columns) or pLuc-1850m (open columns) reporter gene along with 1 μg each of expression plasmid encoding the indicated factor. After incubation for 20 hours, the promoter activity was measured as described in “Materials and methods.” Relative luciferase activity is expressed in arbitrary units, and the data represent the mean of 3 independent experiments with an SE bar.