Cell-cycle analysis of U266 cells cultured with BMP-2.

U266 cells were cultured in the absence or presence of 50 ng/mL of BMP-2 for 0, 4, 24, and 48 hours and then stained with propidium iodide. DNA content was analyzed by means of flow cytometry. G1, S, and G2/M indicate cell phases. Apo indicates apoptotic cells.