Analysis of retinoid receptor expression in D⁺ cells treated for 24 hours with LiCl, ATRA, or their combination.

(A) Western blot analysis of RARα. (B) Northern blot analysis of RARα. The blot was probed for GAPD to show approximately equal loading of RNA. (C) Western blot analysis of RXRα. The blot in A was stripped and reprobed with an antibody to RXRα. Loading was standardized by equal cell numbers. Coomassie staining of a duplicate gel shows approximately equal amounts of protein in each lane. C indicates untreated control; L, LiCl (2.5 mmol/L); R, ATRA (3 μmol/L); LR, combination of LiCl and ATRA.