Northern blot analysis of inducible expression of AML1-ETO in founder line #7.

Transgenic mice were generated that were positive either for pUHD–AML1-ETO or for MMTV-tTA and pUHD–AML1-ETO. Mice from founder line #7 were killed, and total RNAs were isolated from various tissues. Total RNA (10 μg) was loaded on each lane. The RNA was then transferred to a nylon membrane and hybridized sequentially with tTA and ETO probes. The ethidium bromide staining of the 28S ribosomal RNA is presented to show the loading of the RNA samples.