Northern blot analysis of inducible expression of AML1-ETO in transgenic mice.

Transgenic mice were generated that were positive for either pUHD–AML1-ETO or for MMTV-tTA and pUHD–AML1-ETO based on Southern blot analyses. Mice from 3 unique founder lines were killed, and total RNAs were isolated from various tissues. Total RNA (10 μg) was loaded on each lane. The RNA was then transferred to a nylon membrane and hybridized sequentially with fragments of the tTA gene and ETO cDNA. The ethidium bromide staining of the 28S ribosomal RNA is presented to show the loading of the RNA samples.