Fig. 2.
![Fig. 2. Development of IL-3–dependent bone marrow–derived mast cells (BMMCs) is normal in γc− and Jak3− mice. / (A) BMMCs (2 × 105/well) were cultured in the presence of IL-3 at 37°C for 36 hours, and the proliferative responses were evaluated by the addition of [3H] thymidine for the final 12 hours. Data are means ± SD for 5 mice in each group. (B) BMMCs from WT, γc−, and Jak3− mice were stained with anti–c-kit APC, anti-FcγII/III PE, and IgE FITC, as described in “Materials and methods.” Shown are representative FACS profiles for c-kit, FcγII/III, and IgE receptor staining from 5 independent experiments. Dashed lines are FACS profiles of negative controls.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/96/6/10.1182_blood.v96.6.2172/5/h81800149002.jpeg?Expires=1724228006&Signature=XIp6U~BPLo6t6pQr4J93uY~-6~bpzZ8ZnOanZKZAUKyhzrHq8EpJRYclTcmGq6jXUJDfBjoiOIHDWtsZAYaVhw4EFKp~DlPa~-heNGTIhq78UN03nKZYgp2WOz92yecfyUQsXbggTxrBJIGGVgQPdMtVk2rNmpTEXlfGJoOruxINSJi4Mk3Qech13NMb0YNnqt3NGFq1nCB-UuXS3KJIdGMxFlBCVNSJ3zjppqkWS1SEhqrfPg4wLIz4IQ6wt4GAU2OzWHE~-AnYJy6kpdwhSaolXl~83437T~raIa3kR7Wc4Z~gdXWCRL4cBQYwmISE8DFtPwnbw2dU~RTfhVMiuQ__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Development of IL-3–dependent bone marrow–derived mast cells (BMMCs) is normal in γc− and Jak3− mice.
(A) BMMCs (2 × 105/well) were cultured in the presence of IL-3 at 37°C for 36 hours, and the proliferative responses were evaluated by the addition of [3H] thymidine for the final 12 hours. Data are means ± SD for 5 mice in each group. (B) BMMCs from WT, γc−, and Jak3− mice were stained with anti–c-kit APC, anti-FcγII/III PE, and IgE FITC, as described in “Materials and methods.” Shown are representative FACS profiles for c-kit, FcγII/III, and IgE receptor staining from 5 independent experiments. Dashed lines are FACS profiles of negative controls.
