Interaction between the DRIP complex and the retinoid receptors in U937 clones that differ in their PML-RARα expression.
(A) Expression of RARα and fusion PML-RARα proteins in NB4 and U937 subclones by Western blot analysis. Twenty micrograms of the nuclear extracts were run on SDS-PAGE, transferred, and probed with an anti-RARα antibody recognizing its F domain. Positions of RARα and the fusion PML-RARα proteins are indicated. (B) Interaction of the DRIP/TRAP complex with retinoid receptors in U937 subclones (lanes 6-11), HL-60 cells (lanes 4, 5). (C) Direct interaction between the PML-RARα protein and the DRIP205. In vitro translated35S-labeled PML-RARα and mutant PML-RARα M4 were incubated with purified GST-DRIP205 in the absence (−) and presence (+) of 1 μmol/L or 10 μmol/L t-RA (lanes 3, 4, 7, 8). GST alone was included as the negative control (lanes 1, 5).
