Ligand-inducible interaction of the DRIP/TRAP complex to RXR/RAR heterodimer in NB4 and 3 RA-resistant NB4 subclones.

Nuclear extracts (1.2 mg) prepared from different cell lines were incubated with 10 to 20 μg of the immobilized GST-RXR LBD/RAR LBD in the absence (−) or presence (+) of 5 μmol/L t-RA. GST alone was used as a negative control (lane 1). The approximate molecular weights of the interacting proteins are indicated at right.