Fig. 1.
Vβ3, Vβ14, and Vβ17 expression on positively selected primary human CD4 T cells and their proliferation after antigenic stimulation.
(A) Cells were positively selected with Vβ-specific mouse antibodies, stained with a PE-labeled antimouse antibody, and analyzed on a FACSsort. The filled histogram shows a staining of nonselected cells with the secondary antibody alone. (B) The proliferative response of primary CD4+ T cells was determined at day 4 and 7 in duplicates. Cells were incubated over night with 0.037 MBq (1 μCi)3H-thymidine and harvested on glass fiber filters. These filters were measured with a Trace 96.
