Fig. 8.
Effect of L-NMMA on the expression of GATA-2.
EMSA was performed using 1.5 μg of protein from Hep3B cells under normoxia (lanes 2-6 and 13-17) and hypoxia (lanes 7-11 and 18-22), from Hep3B cells incubated with 10−5 mol/L L-NMMA (lanes 3, 8, 14, and 19), 10−4 mol/L L-NMMA (lanes 4, 9, 15, and 20), 10−3 mol/L L-NMMA (lanes 5, 10, 16, and 21), and 10−2 mol/L L-NMMA (lanes 6, 11, 17, and 22) for 1 hour. The dot at the left indicates the position of the GATA-2 transcription factor. A total of 25 ng (0.5 μL: 12.5-fold molar excess) of competitor DNA was added to each reaction mixture (lanes 13-22).
