Constructs used in this study.

(A) The GATA-binding site in the Epo 5′ promoter region (boxed) and repeated CACCC elements (underlined). (B) Diagrams of the reporter construct, the wild-type (Pwt) and the mutated GATA (Pm7) used in this study. The pEPLuc reporter construct is shown in the center. Shown above is the 144-bp insert from the Epo promoter. The mutation is indicated by underlining. The Epo enhancer contains an HIF-1 binding site. (C) Diagrams of the reporter constructs, Δ18pXP2, Δ18m7pXP2, used in this study. The mutation is indicated by underlining.