Fig. 5.
Fig. 5. Analysis, sorting windows, and purities of Lin− marrow cells from primary recipients at 8 months posttransplantation. / The primary recipients were the 7 mice presented in Figure 4 that had been transplanted with CD34+ G-CSF–mobilized cells. (A) Dotted and solid lines represent cells stained with isotype-matched immunoglobulin and Ly-5.1-specific antibody, respectively. A total of 13.2% of the Lin− marrow cells of the primary recipients were donor-derived (Ly-5.1) cells. R8 and R9 describe sorting regions used for preparation of Ly-5.1 CD34− and Ly-5.1 CD34+ cells to be transplanted into secondary recipients. The ratio of the cells in R8 to R9 was approximately 1:1. (B) Results of analysis of the purity of the sorted Ly-5.1 CD34− and Ly-5.1 CD34+ bone marrow cells.

Analysis, sorting windows, and purities of Lin− marrow cells from primary recipients at 8 months posttransplantation.

The primary recipients were the 7 mice presented in Figure 4 that had been transplanted with CD34+ G-CSF–mobilized cells. (A) Dotted and solid lines represent cells stained with isotype-matched immunoglobulin and Ly-5.1-specific antibody, respectively. A total of 13.2% of the Lin− marrow cells of the primary recipients were donor-derived (Ly-5.1) cells. R8 and R9 describe sorting regions used for preparation of Ly-5.1 CD34− and Ly-5.1 CD34+ cells to be transplanted into secondary recipients. The ratio of the cells in R8 to R9 was approximately 1:1. (B) Results of analysis of the purity of the sorted Ly-5.1 CD34− and Ly-5.1 CD34+ bone marrow cells.

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