Sorting regions used for preparation of c-kit⁺ Sca-1⁺ CD34⁻ and c-kit⁺ Sca-1⁺ CD34⁺ cell populations.

First, Lin⁺ cells were removed from mononuclear PB cells using immunomagnetic beads. The Lin⁻ cells were then enriched for c-kit⁺ Sca-1⁺ cells by using R3 window and again sorted on the basis of CD34 expression by using R4 and R5 windows.