Actin-based function of patient neutrophils compared to normal neutrophils.

(A) Chemotaxis in a modified Boyden chamber in response to fMLP and IL-8. The number of neutrophils migrating through the filter was determined by counting 5 random 400× microscope fields. Data are expressed as mean number of cells per field ± SEM. *P = .01; **P = .05. (B) Phagocytosis of IgG-coated erythrocytes unstimulated and in response to fMLP. **P = .05, fMLP vs no agonist (patient);^†P = .001 patient vs normal. (C) Neutrophil rolling on the l-selectin ligand, GlyCAM-1. Tethered and rolling cells were scored and averaged in 10 to 12 different fields of the GlyCAM-1–coated plate in the second minute of the rolling assay. Data shown represent the average number of tethered cells, ± SEM, observed using 2 independently isolated cell preparations and 2 independently prepared GlyCAM-1–substituted chamber surfaces. *P = .01. (D) Time course of neutrophil adhesion to fibrinogen. The number of adherent neutrophils was determined by counting 5 random 200× microscope fields. **P = .05 only at the 30-minute time point; P > .05 (NS) all other points.