Apoptosis and caspase-3 activation following oligodeoxynucleotide delivery to PMA-treated U937 cells.

(A) Representative flow cytometry traces of PI-stained nuclei, cells treated with Mcl AS8 5.9.5 (dark-shaded trace), SL-O (light-shaded trace) for 18 hours, and non-PMA–treated cells (open trace). (B) Apoptosis 18 hours after oligodeoxynucleotide delivery. The data are the mean plus or minus SD of 3 separate experiments. Significant difference from cells treated only by SL-O is represented by a dagger (P ≤ .05) or an asterisk (P ≤ .01). (C) Western blot showing Mcl-1 protein and caspase-3 (where p32 equals procaspase-3, and p17 equals a large active subunit) 4 hours after AS delivery. The data are representative of 3 experiments.