Induction of TNF-α and Mcl-1 in PMA-treated U937 cells: specificity of AS treatments.

Oligodeoxynucleotides (Mcl AS8 5.9.5, TNF AS 6.8.5, and the inverted controls) were delivered into PMA-stimulated U937 cells stimulated for 3 hours. Mcl-1 and TNF-α mRNA were analyzed by Northern blotting. The data are shown as the mean plus or minus SD of 3 separate experiments. Significant difference from cells treated only with SL-O is indicated by an asterisk (P < .001).