Growth factor withdrawal induces p27 in M07 cells.

M07 cells were cultured for 18 hours with or without GM-CSF. (A) Proportion of S-phase cells in the presence or absence of GM-CSF. Data represent the mean ± SE of 3 independent examinations. (B) Relative changes in expression levels of p27, cyclin D2, cyclin D3, cyclin E, CDK2, and PCNA after growth factor withdrawal for 18 hours. Lysates were prepared and subjected to Western blot analysis using anti-p27, anticyclin D3, anticyclin E, anticyclin D2, anti-PCNA, and anti-CDK2 antibodies. Data shown are representative of at least 5 independent examinations.