Inhibition Cvx- or collagen-induced platelet activation by rhusGPVI:Fc.
(A) Trace a: platelets were activated by 100 pmol/L Cvx; trace b: platelet suspension was incubated with 1 μg GPVI:Fc for 2 minutes before the addition of Cvx; traces c and d: Cvx was preincubated with 0.25 μg and 0.5 μg GPVI:Fc for 2 minutes, respectively, before the addition to platelets. (B) Trace a: platelets were activated by collagen type I; trace b: platelets were preincubated with 5 μg GPVI:Fc for 2 minutes before the addition of collagen; traces c to e: collagen was preincubated with 1 μg, 2.5 μg, and 5 μg GPVI:Fc, respectively, for 2 minutes before the addition to platelets. 14C 5-HT–labeled washed platelets were used. The percentage of14C 5-HT release measured in each condition is indicated.
