Fig. 6.
Fig. 6. Adhesion of cells expressing rhusGPVI or rmGPVI to immobilized Cvx or collagen. / BSA, Cvx, or collagen type I (ColI) were immobilized on microtitration plates. (A) rhuGPVI transduced or control U937 cells or (B) rmGPVI transduced or control FDC-P1 cells were labeled with 51Cr and incubated for 60 minutes in the wells. After aspiration of the nonbound cells and washing, radioactivity associated to the wells was counted to determine the adherent cell number. The results are expressed as the percentage of the cells added to the wells and are the mean plus or minus SEM of 3 values. The control cells are indicated by the open bars, and the GPVI-expressing cells are indicated by the darkened bars.

Adhesion of cells expressing rhusGPVI or rmGPVI to immobilized Cvx or collagen.

BSA, Cvx, or collagen type I (ColI) were immobilized on microtitration plates. (A) rhuGPVI transduced or control U937 cells or (B) rmGPVI transduced or control FDC-P1 cells were labeled with 51Cr and incubated for 60 minutes in the wells. After aspiration of the nonbound cells and washing, radioactivity associated to the wells was counted to determine the adherent cell number. The results are expressed as the percentage of the cells added to the wells and are the mean plus or minus SEM of 3 values. The control cells are indicated by the open bars, and the GPVI-expressing cells are indicated by the darkened bars.

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