American Society of Hematology

Fig. 3.

$Fig. 3. Phenotypic characterization of primitive human FB subsets capable of in vitro progenitor and repopulating capacity in NOD/SCID mice. / (A) Representative analysis of cell surface CD34 and CD38 by multiparameter flow cytometry of human FB cells depleted for cells expressing lineage commitment markers (Lin−). Subpopulations of CD34+CD38−Lin−, CD34+CD38+Lin−, and CD34−CD38−Lin− were gated R1, R2, and R3, respectively, and isolated for functional analysis using in vitro and in vivo assays. (B) CFC capacity was assessed in subfractions indicated and represented as the number of CFCs per 1000 purified cells. Values are the mean and SEM of determinations from up to 5 separate FB samples. (C) Summary of the level of human cell engraftment in the BM of NOD/SCID mice transplanted (n = 25) with purified cell fractions from 6 independent FB samples. Each symbol represents a single NOD/SCID recipient.$

Phenotypic characterization of primitive human FB subsets capable of in vitro progenitor and repopulating capacity in NOD/SCID mice.

(A) Representative analysis of cell surface CD34 and CD38 by multiparameter flow cytometry of human FB cells depleted for cells expressing lineage commitment markers (Lin⁻). Subpopulations of CD34⁺CD38⁻Lin⁻, CD34⁺CD38⁺Lin⁻, and CD34⁻CD38⁻Lin⁻ were gated R1, R2, and R3, respectively, and isolated for functional analysis using in vitro and in vivo assays. (B) CFC capacity was assessed in subfractions indicated and represented as the number of CFCs per 1000 purified cells. Values are the mean and SEM of determinations from up to 5 separate FB samples. (C) Summary of the level of human cell engraftment in the BM of NOD/SCID mice transplanted (n = 25) with purified cell fractions from 6 independent FB samples. Each symbol represents a single NOD/SCID recipient.

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