gp120 binding and HIV-1 coreceptor function of CCR5 natural mutants.

(A) 293T cells transfected with wtCCR5 or mutants were tested for their ability to bind [¹²⁵I]-gp120 from the M-tropic HIV-1 strain JRFL in the presence of soluble CD4 (100 nmol/L). The data were normalized for the specific binding on wtCCR5 (100%) after deduction of nonspecific binding (bound [¹²⁵I]-gp120 on cells transfected with the pcDNA3 vector alone). Results represent the mean and SEM of four experiments performed separately. (B) The ability of GFP reporter viruses pseudotyped with R5 HIV-1 Envs (JRFL) to infect 293T cells expressing CD4 and CCR5 natural mutants was assayed. The data were normalized to the luminescence obtained with wtCCR5 (100%) and represent the mean and SEM of two independent experiments.