Effect of allergen challenge on eosinophil levels in Peyer's patches.
Eosinophils were quantitated in the Peyer's patches of wild-type mice subjected to intranasal (A) or oral (B) allergen or placebo challenge. Eosinophils in the Peyer's patches were determined by anti-MBP immunostaining and the results are expressed as MBP per Peyer's patches area (%), based on morphometric analysis. The results are represented as mean ± SEM (n = 9-12). Statistical significance of experimental groups was analyzed using the unpaired Studentt test. Normalization of Peyer's patch eosinophils per mm2 resulted in 0.32 ± 0.21 and 16.1 ± 4.6 (mean ± SEM, n = 6) eosinophils/mm2 for saline andA fumigatus antigen–challenged mice, respectively; and 0.76 ± 0.42 and 15.0 ± 8.7 (mean ± SEM, n = 7-10) for placebo- and OVA-challenged mice, respectively.
