Fig. 4.
Fig. 4. LS174T cell adhesion to surface-adherent platelets under conditions of flow (0.8 dyn/cm2)—effects of antibodies and peptides. / Closed bars represent the stable adherent cells (stationary for > 10 seconds) counted at the end of the 10-minute experiment, whereas open bars represent the total interacting cells counted throughout the entire 10-minute experiment. Immobilized platelets were treated with antiplatelet agents during the 10-minute thrombin incubation. Saturating concentrations were also maintained in the flow buffer only for the GRGDSP peptide, anti-vWf polyclonal antibody, and antifibrinogen (NYB-4.2) mAb. HIP8 (blocking αIIbβ3 function); P2 (blocking anti-αIIb mAb); SZ21 (blocking anti-β3mAb).*, § P < .05 with respect to no-treatment control. Values are mean ± SEM of 3-31 experiments.

LS174T cell adhesion to surface-adherent platelets under conditions of flow (0.8 dyn/cm2)—effects of antibodies and peptides.

Closed bars represent the stable adherent cells (stationary for > 10 seconds) counted at the end of the 10-minute experiment, whereas open bars represent the total interacting cells counted throughout the entire 10-minute experiment. Immobilized platelets were treated with antiplatelet agents during the 10-minute thrombin incubation. Saturating concentrations were also maintained in the flow buffer only for the GRGDSP peptide, anti-vWf polyclonal antibody, and antifibrinogen (NYB-4.2) mAb. HIP8 (blocking αIIbβ3 function); P2 (blocking anti-αIIb mAb); SZ21 (blocking anti-β3mAb).*, § P < .05 with respect to no-treatment control. Values are mean ± SEM of 3-31 experiments.

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