LPS dose response for Mφ-mediated release of intracellular iron from target cells during acute GVH.

Target cell release of ⁵⁹Fe (○) and ⁵¹Cr (▵) mediated by Mφ from normal B6AF₁ mice and release of ⁵⁹Fe (●) and ⁵¹Cr (▴) mediated by Mφ from acute GVHD B6AF₁ mice transplanted 14 days previously with 60 × 10⁶ B6 cells. Mφ-mediated radioisotope release was determined using ⁵⁹Fe,⁵¹Cr dual-labeled MDW4 cells in an 18-hour assay as described in “Materials and methods.” Each value represents the mean ± SD of 4 replicates of pooled Mφ effector cells. Similar results were obtained in 2 separate experiments.