Detection of WT1-specific antibodies in sera of patients with AML.

Sera of patients with AML were used in a 1:500 dilution. Lanes 1, 3, and 5 show the molecular weight marker (MWM); lanes 2, 4, and 6 show the N-terminus portion of the TRX-WT1 protein. Primary antibodies used were WT180 in lane 2, sera of a healthy donor in lane 4, and sera of a patient with AML in lane 6.