Glutaraldehyde fixation of bone marrow stromal cells does not alter chemotherapy protection.

Confluent stromal cells were treated with 2% glutaraldehyde for 5 minutes and were thoroughly rinsed before the addition of JM-1 cells. Matched control stromal cells were cultured in medium alone. JM-1 cells cultured on control or glutaraldehyde-treated stromal cells were exposed to 5 μmol/L VP-16 or 1.0 μg/mL Ara-C. Chemotherapy-treated JM-1 cells were also evaluated in medium alone. After 48 and 72 hours of culture, JM-1 cells were collected, and viability of triplicate samples were evaluated by trypan blue exclusion.