HPC commitment reprogramming by PML/RARα: single-cell analysis.

(A) Scheme of sibling HPCs generation, infection and analysis. (B) Morphologic analysis of the clones generated by single sibling HPC infected with control (GFP) or PML/RARα vector. (left panel) E, erythroid clones; E + G mixed clones with erythroid and myeloid cells, mostly represented by promyelocytes; G, granulocytic clones, primarily promyelocytic. (right panel) Differentiation levels of erythroid clones, as evaluated from percentages of orthochromatic erythroblasts. Mean ± SD values from 3 experiments in which at least 20 sibling colonies/vector were studied.