Fig. 3.
Unilineage granulopoietic (G) proliferation and differentiation of HPC transduced with the indicated vector.
These findings are from 1 of 5 representative experiments. (A) Cell growth induced by G HGF at standard concentration,21 1:100 or 1:1000 dilutions. (B) Cell differentiation as assessed by morphologic analysis of liquid cultures in the presence of standard concentrations of G HGF, standard concentrations of G HGFs with the addition of 10−6 mol/L RA, or 1:100 diluted G HGF. As described in “Materials and Methods,” the HPCs were isolated and stimulated with growth factors on days −4 and −3, infected on day −2 and −1, and FACS purified on day 0.
