Fig. 2.
Fig. 2. PML/RARα induces promyelocytic differentiation of HPC. / (A) The morphology of the cells was analyzed on cytospin slides stained with May-Grünwald-Giemsa. (first left panel) HPCs stimulated with IL-3, FLT3 ligand, and stem cell factor (see “Materials and methods”) before retroviral infection. (right panels) HPCs transduced with the control (GFP) or the indicated fusion protein vector. Mature G cells comprise metamyelocytes, myelocytes, and granulocytes. Mean ± SD values from 5 experiments. The percentage of CD34+cells in the different samples is analyzed by FACS and is indicated above the columns. Single and double asterisks above the columns indicate statistical significance (P < .0001) of the differences in GFP vs. PML/RARα cells and PML/RARα vs. PML/RARα-AHT cells, respectively, as calculated from Student 2-tailed t test. (B) Representative cytospins of the samples described in A.

PML/RARα induces promyelocytic differentiation of HPC.

(A) The morphology of the cells was analyzed on cytospin slides stained with May-Grünwald-Giemsa. (first left panel) HPCs stimulated with IL-3, FLT3 ligand, and stem cell factor (see “Materials and methods”) before retroviral infection. (right panels) HPCs transduced with the control (GFP) or the indicated fusion protein vector. Mature G cells comprise metamyelocytes, myelocytes, and granulocytes. Mean ± SD values from 5 experiments. The percentage of CD34+cells in the different samples is analyzed by FACS and is indicated above the columns. Single and double asterisks above the columns indicate statistical significance (P < .0001) of the differences in GFP vs. PML/RARα cells and PML/RARα vs. PML/RARα-AHT cells, respectively, as calculated from Student 2-tailed t test. (B) Representative cytospins of the samples described in A.

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