Northern blot analysis showing up-regulation of tartrate-resistant Acp 5 and chitotriosidase in Gaucher spleen.

(A) The up-regulation of tartrate-resistant Acp 5 and (B) chitotriosidase in Gaucher spleen are depicted. The filters were also later hybridized to radio-labeled β-actin, shown in panel A, and glyceraldehyde phosphate dehydrogenase control cDNA probes, shown in panel B. The figure is a radioautograph and shows hybridization to samples of RNA obtained from control and Gaucher disease tissue independent from that used in the generation of the Gaucher-specific cDNA library by suppression subtraction hybridization.