Enrichment of genes differentially expressed in Gaucher tissue.

(A) Suppression subtraction hybridization. The figure shows ethidium bromide–stained cDNA fragments resolved by electrophoresis in a 2% wt/vol agarose gel. (B) Southern blot analysis of subtracted (enriched) and unsubtracted Gaucher spleen cDNA library hybridized with³²P-labeled human tartrate-resistant Acp 5 cDNA. The principal hybridization species correspond to the predictedRsaI fragments of 423 and 590 bp.23 (C) Southern blot of subtracted (enriched) and unsubtracted Gaucher spleen cDNA library hybridized with ³²P-labeled human chitotriosidase cDNA. The principal hybridizing species correspond to the predicted RsaI fragments of 123, 423, and 488 bp.21