Fluorescence microscopy of transduced cells.

Monocytes were cultivated with GM-CSF or with GM-CSF and IL-4 to generate Mo macrophages (A) and DCs (B and C), respectively. On day 7, cells were transduced with HIV-1ΔEN V3 (A and B) or with HIV-1ΔEN (C) at a MOI of 5 and incubated further in cyokine(s) supplemented medium. On day 5 after transduction, cells were analyzed by fluorescence microscopy. DCs were either analyzed directly (immature DCs; B) or treated with LPS to generate mature DCs (C) before analysis by fluorescence microscopy.