American Society of Hematology

Fig. 5.

$Fig. 5. Syntaxin 2 and syntaxin 4 localize to granules and OCS. / . For immunoelectron microscopy, platelets were fixed, dehydrated in a series of ethyl alcohols, and embedded in LR White resin. Samples were thin sectioned, incubated with primary antisyntaxin antibodies (panel B for syntaxin 2 and C for syntaxin 4) or a preimmune immunoglobulin fraction (panel A). The grids were then incubated with gold-conjugated goat antirabbit secondary antibodies 20 nm particle for syntaxin 2, 10 nm particle for syntaxin 4, and counterstained with uranyl acetate and lead citrate. Arrows indicate labeling of the granule. Arrowheads refer to gold particles found on the OCS. Bars equal 0.5 μm.$

Syntaxin 2 and syntaxin 4 localize to granules and OCS

. For immunoelectron microscopy, platelets were fixed, dehydrated in a series of ethyl alcohols, and embedded in LR White resin. Samples were thin sectioned, incubated with primary antisyntaxin antibodies (panel B for syntaxin 2 and C for syntaxin 4) or a preimmune immunoglobulin fraction (panel A). The grids were then incubated with gold-conjugated goat antirabbit secondary antibodies 20 nm particle for syntaxin 2, 10 nm particle for syntaxin 4, and counterstained with uranyl acetate and lead citrate. Arrows indicate labeling of the granule. Arrowheads refer to gold particles found on the OCS. Bars equal 0.5 μm.

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