Anti-NSF antibody inhibits lysosome secretion.

Panel A: SLO-permeabilized platelets were incubated with increasing amounts of 2E5 (monoclonal antibody against NSF, 0-0.32 mg/mL) and of a nonspecific mouse IgG (0-0.32 mg/mL). The Ca⁺⁺-stimulated release of hexosaminidase was measured and normalized to the control (no addition of 2E5), which was set at 100%. Panel B: SLO permeabilized platelets were incubated on ice for 30 minutes with buffer (control), with 80 μg/mL 2E5, with 80 μg/mL that had been preincubated with 0.75 mg/mL recombinant NSF, and with 0.75 mg/mL recombinant NSF alone. Platelets were activated with 10 μmol/L Ca⁺⁺ and the release of hexosaminidase was measured and normalized to the control group.