Fig. 9.
Fig. 9. Comparison of results of cotransduction of ALL (Nalm-6, ALL 1) and AML (ML-1, AML 1) leukemia cells with the separate pHR-CD80 and pHR-GM-CSF vectors and results with the bicistronic pHR-GM/CD vector. / (A) Frequency of CD80+ nontransduced (mock-transduced [M]) cells, cells transduced with pHR-GM-CSF and pHR-CD80 at a multiplicity of infection (MOI) of 1 for each (G+C), and cells transduced with the pHR-GM/CD vector at an MOI of 1. (B) Relative fluorescence of cells in the CD80+ gate. (C) GM-CSF expression measured as picograms per 106 cells per 24 hours.

Comparison of results of cotransduction of ALL (Nalm-6, ALL 1) and AML (ML-1, AML 1) leukemia cells with the separate pHR-CD80 and pHR-GM-CSF vectors and results with the bicistronic pHR-GM/CD vector.

(A) Frequency of CD80+ nontransduced (mock-transduced [M]) cells, cells transduced with pHR-GM-CSF and pHR-CD80 at a multiplicity of infection (MOI) of 1 for each (G+C), and cells transduced with the pHR-GM/CD vector at an MOI of 1. (B) Relative fluorescence of cells in the CD80+ gate. (C) GM-CSF expression measured as picograms per 106 cells per 24 hours.

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