DNA integration and RNA expression after lentiviral vector transduction of Nalm-6 cells.

(A) Southern blot analysis. The arrows indicate the integrated DNA originated from the transduction with lentiviral vectors. (B) Northern blot analysis; L indicates the LTR promoter–transcribed RNA; C, CMV promoter–transcribed RNA; 1, nontransduced cells; 2, cells transduced with pHR-GM-CSF; 3, cells transduced with pHR-CD80; and 4, cells transduced with pHR-GM/CD. The length of the DNA and RNA molecules is indicated in kilobases.