The RANKL inhibitor OPG blunts OC formation induced by IL-7 stimulated T-cell CM.

Purified monocytes from PBSCs were induced to form OCs by stimulation with one quarter volume (final) of IL-7–stimulated T-cell CM (4 × concentrated) (■), in the presence or absence of the RANKL inhibitor OPG (1 μg/mL). (▪, unstimulated.) After 7 to 10 days in culture, TRAP⁺ multinucleated cells were counted. Data representative of 3 independent experiments. P < .01 with respect to IL-7–stimulated T-cell CM control.