Fig. 3.
Fig. 3. Characterization of human osteoclasts generated by IL-7 stimulation of PBSCs. / The multinuclear cells formed after IL-7 stimulation were analyzed for markers of authentic human OCs (see “Materials and methods”). These cells were found to express tartrate resistant acid phosphatase (TRAP) (A); resorp pits on dentine slices (B); express calcitonin receptors (C), which were competed out by 200-fold excess unlabeled calcitonin (data not shown); express the integrin subunits αV (D) and β3 (E); express pp60c-src (F), mature cathepsin K (G), and react with the anti-OC antibody 121F (H). Nonspecific binding was assessed by IgG isotype control (I). Magnification = 200 × for all panels.

Characterization of human osteoclasts generated by IL-7 stimulation of PBSCs.

The multinuclear cells formed after IL-7 stimulation were analyzed for markers of authentic human OCs (see “Materials and methods”). These cells were found to express tartrate resistant acid phosphatase (TRAP) (A); resorp pits on dentine slices (B); express calcitonin receptors (C), which were competed out by 200-fold excess unlabeled calcitonin (data not shown); express the integrin subunits αV (D) and β3 (E); express pp60c-src (F), mature cathepsin K (G), and react with the anti-OC antibody 121F (H). Nonspecific binding was assessed by IgG isotype control (I). Magnification = 200 × for all panels.

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