Western blots of the A antigen on GPs in lysates prepared from group O platelets, normal expressers of A1, and Type I and Type II high expressers of A1.
The platelets were solubilized and separated on a 4%-12% gradient SDS-PAGE under nonreducing conditions. The blots were incubated with monoclonal anti-A (BRIC 145), and the bound antibody was detected with HRP-labeled antimouse IgG and chemiluminescence after 10 seconds (top) and 5 minutes (bottom). (Lane 1) Group O platelets. (Lanes 2 and 3) Type II high-expresser platelets with heavily stained bands of approximately 140 kd and 130 kd at 10-second exposure. (Lanes 4 and 5) Type I high-expresser platelets showing bands with similar mobilities at 5-minute exposure. (Lanes 6 and 7) Normal-expresser A1platelets.
