Expression of apoptosis-related genes during cultures with adiponectin and effect of constitutive

Bcl-2 gene expression on adiponectin-induced apoptosis. (A) M1 cells were cultured in serum-free medium containing 10 μg/mL adiponectin or HSA for the indicated times. Total RNAs were isolated and subjected to Northern blot analyses using the complementary DNAs (cDNAs) of Bcl-2, Bcl-xL,Bax, and β-actin as probes. This figure shows 1 of 3 similar experiments. (B) Total RNAs from parent M1, M1 pcDNA3 (clone 1-3), and M1 hBcl-2 (clone 1-3) were isolated and subjected to Northern blot analyses using the cDNAs of Bcl-2 and β-actin as probes. (C) Triplicate aliquots of parent M1 cells, M1 pcDNA3 clones, or M1 hBcl-2 clones were incubated in serum-free medium containing 10 μg/mL adiponectin for the indicated times. The viability of each clone was determined by trypan blue dye exclusion every 24 hours after initiation of the culture. The results are shown as the mean ± SD values from triplicate cultures. Similar results were obtained in 3 independent experiments.