Differential engraftment kinetics of enriched stem cells isolated from B6 or DBA mice.
Sca-1+c-kit+Lin− bone marrow cells were sorted from B6 (open circles) or DBA (closed circles) marrow and transplanted into lethally irradiated Ly-5 congenic (B6.SJL) or syngeneic DBA recipients, respectively (1000 cells per mouse). Peripheral blood leukocytes (panel A), erythrocytes (panel B), and platelets (panel C) were counted on the indicated days. Shown are the mean ± SEM number of blood cells for pooled data from 2 independent experiments comprising a total of 21 (B6) or 33 (DBA) mice. Error bars not shown are too small for the scale used. DBA values are significantly greater than B6 values (P < .01) at all times after day 9 (day 6 for red cells), except for platelets on day 32. Normal blood counts for B6 and DBA mice, respectively, are leukocytes, 6 to 9 × 103 and 4 to 7 × 103; erythrocytes, 9 to 11 × 106 and 7 to 10 × 106; platelets, 11 to 14 × 105, and 10 to 11 × 105.
