Confocal microscopy and double immunostaining of Flk-1 and PECAM in mouse tissues.

Tissue sections from control mice were processed for immunodetection of Flk-1 and PECAM. High-power view of optical sections through the heart (A-C), liver (D-F), and skeletal muscle (G-I). Flk-1 immunofluorescence is shown on the left (A, D, G), and PECAM immunofluorescence is shown in the middle (B, E, H). The 2 images are combined on the right (C, F, I). Bar, 100 μm.