The amino terminal region of FANCA is required for FANCG binding.

(A) Schematic representation of wild-type (wt) and truncated mutant forms of FANCA. The two indicated mutant FANCA polypeptides contain an HA-epitope tag at their amino terminus. Results of binding studies are summarized as (+) and (−). NLS, nuclear localization signal; Lz, leucine zipper. (B) COS cells were cotransfected with the indicated FANCA cDNA mutants and the full-length FANCG cDNA. Immunoprecipitation (lanes 1-6) was performed with an anti-FANCG antiserum (upper blot) and an anti-HA antibody (middle blot). Immunoblotting was done with an anti-HA monoclonal antibody or with an anti-FANCG antiserum, as indicated. WCE denotes whole cell extract.