Phenotype of BM-LC.

Bone marrow cells were harvested from CD8 wild-type mice and cultured for 13 days at 1 × 10⁵ cells/mL in CM containing GM-CSF, TGF-β, and SCF and then cultured in the presence of TNF-α and GM-CSF and IL-4 for 3 additional days. Contour plots show the CD11c versus IA-b profile of the cells obtained at day 16 of culture. Histograms show the phenotypes of the CD11c⁺IA-b⁺-gated cells.