Migratory LC in the LN, but not in the skin, express CD8α.

(A) LC were isolated from skin and immediately analyzed by FACS. Contour plots represent IA-b versus CD11c profiles, and the histograms show the phenotypes of IA-b⁺ CD11c⁺ cells. (B) Four days after skin painting with FITC, DC were isolated from LN using density-gradient centrifugation. Contour plots represent FITC versus CD11c profiles of the DC-enriched population, and the histograms show the phenotypes of the FITC⁺CD11c⁺ gated cells. Dashed lines give the background fluorescence obtained with an isotype control.