American Society of Hematology

Fig. 2.

$Fig. 2. IR-induced GrB expression. / (A) Flow cytometry analysis of IR-induced GrB expression as determined on KG1a, HEL, TF-1, U937, MCF-7, HeLa, and NIHOVCARIII cell lines. Experiments were performed by using flow cytometry with specific anti-GrB MoAb on cells irradiated (black histograms) or not (white histograms). Each histogram is the mean of five different experiments and is expressed relative to nonirradiated cells.*P < .05 (au: arbitrary units). (B) IR-induced subcellular redistribution of GrB in KG1a cells. Localization of GrB after irradiation in KG1a cells was performed by confocal analysis using specific anti-GrB (i) or irrelevant isotopic murine MoAb (ii) (bar: 5 μm). (C) IR-induced GrB expression in nuclear (pellet fraction) and cytoplasmic (supernatant fraction) lysates in KG1a cells. Western blot analysis of nuclear and cytoplasmic fractions from KG1a was performed using MoAb-detecting GrB (32 kDa; diluted 1/200). For each lane, the nuclear and cytoplasmic lysates were loaded with the same number of cell equivalents.$

IR-induced GrB expression.

(A) Flow cytometry analysis of IR-induced GrB expression as determined on KG1a, HEL, TF-1, U937, MCF-7, HeLa, and NIHOVCARIII cell lines. Experiments were performed by using flow cytometry with specific anti-GrB MoAb on cells irradiated (black histograms) or not (white histograms). Each histogram is the mean of five different experiments and is expressed relative to nonirradiated cells.*P < .05 (au: arbitrary units). (B) IR-induced subcellular redistribution of GrB in KG1a cells. Localization of GrB after irradiation in KG1a cells was performed by confocal analysis using specific anti-GrB (i) or irrelevant isotopic murine MoAb (ii) (bar: 5 μm). (C) IR-induced GrB expression in nuclear (pellet fraction) and cytoplasmic (supernatant fraction) lysates in KG1a cells. Western blot analysis of nuclear and cytoplasmic fractions from KG1a was performed using MoAb-detecting GrB (32 kDa; diluted 1/200). For each lane, the nuclear and cytoplasmic lysates were loaded with the same number of cell equivalents.

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