American Society of Hematology

Fig. 1.

$Fig. 1. GrB expression in leukemic cell lines. / (A) Flow cytometry analysis of GrB expression as determined on KG1a, HEL, TF-1, U937, Jurkat, and activated peripheral blood lymphocytes (PBL-IL2). GrB expression in PBL-IL2 was assessed as positive control. Experiments were performed by using flow cytometry with specific anti-GrB (dotted line). Each histogram is representative of 4 to 6 different experiments. Irrelevant isotopic murine monoclonal antibody (MoAb) (black line) was used as internal control (au: arbitrary units). (B) Subcellular distribution of GrB in KG1a cells. Localization of GrB in KG1a cells was performed by confocal analysis using specific anti-GrB (i) or irrelevant isotopic murine MoAb (ii) (bar: 5 μm). (C) GrB in nuclear (pellet fraction) and cytoplasmic (supernatant fraction) lysates in KG1a cells. Western blot analysis of nuclear and cytoplasmic fractions from KG1a was performed by using MoAb-detecting GrB (32 kDa; diluted 1/200). For each lane, the nuclear and cytoplasmic lysates were loaded with the same number of cell equivalents.$

GrB expression in leukemic cell lines.

(A) Flow cytometry analysis of GrB expression as determined on KG1a, HEL, TF-1, U937, Jurkat, and activated peripheral blood lymphocytes (PBL-IL2). GrB expression in PBL-IL2 was assessed as positive control. Experiments were performed by using flow cytometry with specific anti-GrB (dotted line). Each histogram is representative of 4 to 6 different experiments. Irrelevant isotopic murine monoclonal antibody (MoAb) (black line) was used as internal control (au: arbitrary units). (B) Subcellular distribution of GrB in KG1a cells. Localization of GrB in KG1a cells was performed by confocal analysis using specific anti-GrB (i) or irrelevant isotopic murine MoAb (ii) (bar: 5 μm). (C) GrB in nuclear (pellet fraction) and cytoplasmic (supernatant fraction) lysates in KG1a cells. Western blot analysis of nuclear and cytoplasmic fractions from KG1a was performed by using MoAb-detecting GrB (32 kDa; diluted 1/200). For each lane, the nuclear and cytoplasmic lysates were loaded with the same number of cell equivalents.

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